Welcome to OligoWalk

OligoWalk is an online sever calculating thermodynamic features of sense-antisense hybidization. It predicts the free energy changes of oligonucleotides binding to a target RNA. It can be used to design efficient siRNA targeting a given mRNA sequence. The source code of OligoWalk for siRNA design can be downloaded from here.
The efficient siRNA selection method is described in a published paper (link). More references are listed in the Help page.

The server has been tested on Firefox 2 and Internet Explorer 7.

Target RNA Sequence
*Sequence name
*Primary sequence
paste the sequence here
or upload the sequence file (file format: fasta)
*Email address

Do not use file uploading, please paste your sequence above for

The fields indicated with an asterisk (*) are required.