16  ENERGY = 1.3  Reverse Primer
    1 C       0    2    0    1
    2 C       1    3    0    2
    3 T       2    4    0    3
    4 C       3    5    0    4
    5 C       4    6    0    5
    6 T       5    7    0    6
    7 C       6    8    0    7
    8 C       7    9    0    8
    9 T       8   10   15    9
   10 C       9   11    0   10
   11 C      10   12    0   11
   12 T      11   13    0   12
   13 C      12   14    0   13
   14 C      13   15    0   14
   15 A      14   16    9   15
   16 G      15    0    0   16