Initializing nucleic acids...done. Applying constraints...Warning: Invalid nucleobase positions in SHAPE file Pipeline_SINEC_Cf_80_amplicon.shape: [81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155]. (Sequence length is 80.) done. Folding single strand... 0% [= ] / 6% [==== ] - 12% [======= ] \ 18% [========== ] | 25% [============= ] / 31% [================ ] - 37% [=================== ] \ 43% [====================== ] | 50% [========================== ] / 56% [============================= ] - 62% [================================ ] \ 68% [=================================== ] | 75% [====================================== ] / 81% [========================================= ] - 87% [============================================ ] \ 93% [=============================================== ] | done. Writing output ct file...done. Single strand folding complete.