Initializing nucleic acids...done. Applying constraints...Warning: Invalid nucleobase positions in SHAPE file Pipeline_SINEC_Cf_PrimerSwitch_amplicon.shape: [190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208]. (Sequence length is 189.) done. Folding single strand... 0% [= ] / 2% [== ] - 5% [=== ] \ 7% [==== ] | 10% [====== ] / 13% [======= ] - 15% [======== ] \ 18% [========== ] | 21% [=========== ] / 23% [============ ] - 26% [============== ] \ 29% [=============== ] | 31% [================ ] / 34% [================== ] - 37% [=================== ] \ 39% [==================== ] | 42% [====================== ] / 44% [======================= ] - 47% [======================== ] \ 50% [========================== ] | 52% [=========================== ] / 55% [============================ ] - 58% [============================== ] \ 60% [=============================== ] | 63% [================================ ] / 66% [================================== ] - 68% [=================================== ] \ 71% [==================================== ] | 74% [====================================== ] / 76% [======================================= ] - 79% [======================================== ] \ 82% [========================================== ] | 84% [=========================================== ] / 87% [============================================ ] - 89% [============================================= ] \ 92% [=============================================== ] | 95% [================================================ ] / 97% [================================================= ] - done. Writing output ct file...done. Single strand folding complete.