Welcome to the AllSub Web Server

The AllSub server takes a nucleic acid sequence, either DNA or RNA, and predicts its lowest free energy conformation and a set of low free energy structures. AllSub is able to predict all lowest free energy structures within a small difference of the lowest free energy structure. These structures represent the most populated structures in the Boltzmann ensemble. They also represent alternative possible lowest free energy structures because of uncertanties in our estimated folding free energy changes. Note that this calculation may take a significant amount of time, depending both on the size of the sequence and the number of suboptimal structures generated.

If you plan to leave this page while waiting for calculations to complete, please make sure you have entered a valid email address below. Leaving this page without entering a valid email address will render results inaccessible.

If you need specific help using the AllSub server, please click here.

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RNAstructure tools have been updated to version which includes several improvements.
Please contact us with any questions or issues regarding this update.

Select Sequence Input:

Select Sequence File:

- OR -
Enter your sequence title and content below (replaces upload if present).
Please enter nucleotides only, no headers or comments in content.
Valid nucleotides are A, C, G, T, U, and X (unknown nucleotide). Lower case nucleotides are forced single stranded in calculation.

Sequence Title: Sequence: (Click here to add an example sequence to the box.)

Select Nucleic Acid Type:

DNA:
RNA:

Select AllSub Options:

Default Data If a default value is left blank, the value is treated as if it was not changed at all. Maximum % Energy Difference: Absolute Energy Difference: Temperature (K):

Optional Data Select Folding Constraints File:

Designate Address to Email Results:

Address to Email Results:

RNAstructure

Web Servers for RNA Secondary Structure Prediction

Welcome to the AllSub Web Server

Select Sequence Input:

Select Nucleic Acid Type:

Select AllSub Options:

Designate Address to Email Results: