Data Arangement: Y ------------------ (X) A C G U ------------------ 5' ==> 3' AX AY 3' <== 5' (A) . . . . (C) . . . . (G) . . . . (U) -0.7 -0.1 -0.7 -0.1 -------------------------------- STACKING ENERGIES : Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' AX AX AX AX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . . . . . -0.9 . . . . . . . . . . . . . . -2.2 . . . . . . . . . . . . . . -2.1 . -0.6 . . . . . . . . . . . . -1.1 . -1.4 . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' CX CX CX CX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . -2.1 . . . . . . . . . . . . . . -3.3 . . . . . . . . . . . . . . -2.4 . -1.4 . . . . . . . . . . . . -2.1 . -2.1 . . . . . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' GX GX GX GX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . -2.4 . . . . . . . -1.3 . . . . . . -3.4 . . . . . . . -2.5 . . . . . . -3.3 . -1.5 . . . . . -2.1 . -0.5 . . . . -2.2 . -2.5 . . . . . -1.4 . +1.3 . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' UX UX UX UX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . -1.3 . . . . . . . -1.0 . . . . . . -2.4 . . . . . . . -1.5 . . . . . . -2.1 . -1.0 . . . . . -1.4 . +0.3 . . . . -0.9 . -1.3 . . . . . -0.6 . -0.5 . . . . .