Data tables for asymmetric interior loops of size 3 Free energies at 37 degrees for RNA Data arrangement: Y ------------------ (X) A C G U ------------------ 5' --> 3' X A A U U YA 3' <-- 5' (A) . . . . (C) . . . . (G) . . . . (U) . . . . Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X A A A C A G A U A G A U U U U G U C U A U U U G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.9 3.7 3.1 5.5 3.2 3.0 2.4 4.8 3.2 3.0 2.4 4.8 3.9 3.7 3.1 5.5 3.9 3.7 3.1 5.5 3.9 3.7 3.1 5.5 3.8 3.7 5.5 3.7 3.1 3.0 4.8 3.0 3.1 3.0 4.8 3.0 3.8 3.7 5.5 3.7 3.8 3.7 5.5 3.7 3.8 3.7 5.5 3.7 3.2 5.5 2.3 5.5 2.5 4.8 1.6 4.8 2.5 4.8 1.6 4.8 3.2 5.5 2.3 5.5 3.2 5.5 2.3 5.5 3.2 5.5 2.3 5.5 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X A A A C A G A U A G A U U U U G U C U A U U U G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.6 3.2 3.1 5.5 2.9 2.5 2.4 4.8 2.9 2.5 2.4 4.8 3.6 3.2 3.1 5.5 3.6 3.2 3.1 5.5 3.6 3.2 3.1 5.5 3.7 4.0 5.5 3.7 3.0 3.3 4.8 3.0 3.0 3.3 4.8 3.0 3.7 4.0 5.5 3.7 3.7 4.0 5.5 3.7 3.7 4.0 5.5 3.7 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 3.7 5.5 2.8 4.8 3.0 4.8 2.1 4.8 3.0 4.8 2.1 5.5 3.7 5.5 2.8 5.5 3.7 5.5 2.8 5.5 3.7 5.5 2.8 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X A A A C A G A U A G A U U U U G U C U A U U U G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 2.5 2.1 1.9 5.5 1.8 1.4 1.2 4.8 1.8 1.4 1.2 4.8 2.5 2.1 1.9 5.5 2.5 2.1 1.9 5.5 2.5 2.1 1.9 5.5 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 2.3 5.5 3.7 5.5 1.6 4.8 3.0 4.8 1.6 4.8 3.0 4.8 2.3 5.5 3.7 5.5 2.3 5.5 3.7 5.5 2.3 5.5 3.7 5.5 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X A A A C A G A U A G A U U U U G U C U A U U U G YU YU YU YU YU YU 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 4.0 3.4 5.5 3.7 3.3 2.7 4.8 3.0 3.3 2.7 4.8 3.0 4.0 3.4 5.5 3.7 4.0 3.4 5.5 3.7 4.0 3.4 5.5 3.7 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 3.2 5.5 2.7 4.8 2.5 4.8 2.0 4.8 2.5 4.8 2.0 5.5 3.2 5.5 2.7 5.5 3.2 5.5 2.7 5.5 3.2 5.5 2.7 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X C A C C C G C U C G C U G U G G G C G A G U G G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.2 3.0 2.4 4.8 2.3 2.2 1.1 4.0 2.4 2.2 1.6 4.0 3.2 3.0 2.4 4.8 3.2 3.0 2.4 4.8 3.2 3.0 2.4 4.8 3.1 3.0 4.8 3.0 2.3 2.2 4.0 2.2 2.3 2.2 4.0 2.2 3.1 3.0 4.8 3.0 3.1 3.0 4.8 3.0 3.1 3.0 4.8 3.0 2.5 4.8 1.6 4.8 1.7 4.0 0.8 4.0 1.7 4.0 0.8 4.0 2.5 4.8 1.6 4.8 2.5 4.8 1.6 4.8 2.5 4.8 1.6 4.8 4.8 4.8 4.8 4.8 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X C A C C C G C U C G C U G U G G G C G A G U G G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 2.9 2.5 2.4 4.8 2.1 1.7 1.6 4.0 2.1 1.7 1.6 4.0 2.9 2.5 2.4 4.8 2.9 2.5 2.4 4.8 2.9 2.5 2.4 4.8 3.0 3.3 4.8 3.0 2.2 2.5 4.0 2.2 2.2 2.5 4.0 2.2 3.0 3.3 4.8 3.0 3.0 3.3 4.8 3.0 3.0 3.3 4.8 3.0 4.8 4.8 4.8 4.8 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 3.0 4.8 2.1 4.0 2.2 4.0 1.5 4.0 2.2 4.0 1.3 4.8 3.0 4.8 2.1 4.8 3.0 4.8 2.1 4.8 3.0 4.8 2.1 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X C A C C C G C U C G C U G U G G G C G A G U G G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 1.8 1.4 1.2 4.8 0.8 0.6 0.4 4.0 1.0 0.6 0.4 4.0 1.8 1.4 1.2 4.8 1.8 1.4 1.2 4.8 1.8 1.4 1.2 4.8 4.8 4.8 4.8 4.8 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 1.6 4.8 3.0 4.8 0.8 4.0 2.2 4.0 0.8 4.0 2.2 4.0 1.6 4.8 3.0 4.8 1.6 4.8 3.0 4.8 1.6 4.8 3.0 4.8 4.8 4.8 4.8 4.8 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X C A C C C G C U C G C U G U G G G C G A G U G G YU YU YU YU YU YU 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 4.8 4.8 4.8 4.8 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 3.3 2.7 4.8 3.0 2.5 1.9 4.0 2.2 2.5 1.9 4.0 2.2 3.3 2.7 4.8 3.0 3.3 2.7 4.8 3.0 3.3 2.7 4.8 3.0 4.8 4.8 4.8 4.8 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 2.5 4.8 2.0 4.0 1.7 4.0 1.2 4.0 1.7 4.0 1.2 4.8 2.5 4.8 2.0 4.8 2.5 4.8 2.0 4.8 2.5 4.8 2.0 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U C U C G C C C A C U C G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.2 3.0 2.4 4.8 2.4 2.2 1.6 4.0 2.5 2.2 2.1 4.0 3.2 3.0 2.4 4.8 3.2 3.0 2.4 4.8 3.2 3.0 2.4 4.8 3.1 3.0 4.8 3.0 2.3 2.2 4.0 2.2 2.3 2.2 4.0 2.2 3.1 3.0 4.8 3.0 3.1 3.0 4.8 3.0 3.1 3.0 4.8 3.0 2.5 4.8 1.6 4.8 1.7 4.0 0.8 4.0 1.7 4.0 0.8 4.0 2.5 4.8 1.6 4.8 2.5 4.8 1.6 4.8 2.5 4.8 1.6 4.8 4.8 4.8 4.8 4.8 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U C U C G C C C A C U C G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 2.9 2.5 2.4 4.8 2.1 1.7 1.6 4.0 2.1 1.7 1.6 4.0 2.9 2.5 2.4 4.8 2.9 2.5 2.4 4.8 2.9 2.5 2.4 4.8 3.0 3.3 4.8 3.0 2.2 2.5 4.0 2.2 2.2 2.5 4.0 2.2 3.0 3.3 4.8 3.0 3.0 3.3 4.8 3.0 3.0 3.3 4.8 3.0 4.8 4.8 4.8 4.8 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 3.0 4.8 2.1 4.0 2.2 4.0 1.3 4.0 2.2 4.0 1.2 4.8 3.0 4.8 2.1 4.8 3.0 4.8 2.1 4.8 3.0 4.8 2.1 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U C U C G C C C A C U C G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 1.8 1.4 1.2 4.8 1.0 0.6 0.4 4.0 1.2 0.6 0.4 4.0 1.8 1.4 1.2 4.8 1.8 1.4 1.2 4.8 1.8 1.4 1.2 4.8 4.8 4.8 4.8 4.8 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 1.6 4.8 3.0 4.8 0.8 4.0 2.2 4.0 0.8 4.0 2.2 4.0 1.6 4.8 3.0 4.8 1.6 4.8 3.0 4.8 1.6 4.8 3.0 4.8 4.8 4.8 4.8 4.8 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U C U C G C C C A C U C G YU YU YU YU YU YU 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 4.8 4.8 4.8 4.8 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 3.3 2.7 4.8 3.0 2.5 1.9 4.0 2.2 2.5 1.9 4.0 2.2 3.3 2.7 4.8 3.0 3.3 2.7 4.8 3.0 3.3 2.7 4.8 3.0 4.8 4.8 4.8 4.8 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 2.5 4.8 2.0 4.0 1.7 4.0 1.2 4.0 1.7 4.0 1.2 4.8 2.5 4.8 2.0 4.8 2.5 4.8 2.0 4.8 2.5 4.8 2.0 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U A U A G A C A A A U A G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.9 3.7 3.1 5.5 3.2 3.0 2.4 4.8 3.2 3.0 2.4 4.8 3.9 3.7 3.1 5.5 3.9 3.7 3.1 5.5 3.9 3.7 3.1 5.5 3.8 3.7 5.5 3.7 3.1 3.0 4.8 3.0 3.1 3.0 4.8 3.0 3.8 3.7 5.5 3.7 3.8 3.7 5.5 3.7 3.8 3.7 5.5 3.7 3.2 5.5 2.3 5.5 2.5 4.8 1.6 4.8 2.5 4.8 1.6 4.8 3.2 5.5 2.3 5.5 3.2 5.5 2.3 5.5 3.2 5.5 2.3 5.5 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U A U A G A C A A A U A G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.6 3.2 3.1 5.5 2.9 2.5 2.4 4.8 2.9 2.5 2.4 4.8 3.6 3.2 3.1 5.5 3.6 3.2 3.1 5.5 3.6 3.2 3.1 5.5 3.7 4.0 5.5 3.7 3.0 3.3 4.8 3.0 3.0 3.3 4.8 3.0 3.7 4.0 5.5 3.7 3.7 4.0 5.5 3.7 3.7 4.0 5.5 3.7 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 3.7 5.5 2.8 4.8 3.0 4.8 2.1 4.8 3.0 4.8 2.1 5.5 3.7 5.5 2.8 5.5 3.7 5.5 2.8 5.5 3.7 5.5 2.8 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U A U A G A C A A A U A G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 2.5 2.1 1.9 5.5 1.8 1.4 1.2 4.8 1.8 1.4 1.2 4.8 2.5 2.1 1.9 5.5 2.5 2.1 1.9 5.5 2.5 2.1 1.9 5.5 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 2.3 5.5 3.7 5.5 1.6 4.8 3.0 4.8 1.6 4.8 3.0 4.8 2.3 5.5 3.7 5.5 2.3 5.5 3.7 5.5 2.3 5.5 3.7 5.5 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U A U A G A C A A A U A G YU YU YU YU YU YU 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 4.0 3.4 5.5 3.7 3.3 2.7 4.8 3.0 3.3 2.7 4.8 3.0 4.0 3.4 5.5 3.7 4.0 3.4 5.5 3.7 4.0 3.4 5.5 3.7 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 3.2 5.5 2.7 4.8 2.5 4.8 2.0 4.8 2.5 4.8 2.0 5.5 3.2 5.5 2.7 5.5 3.2 5.5 2.7 5.5 3.2 5.5 2.7 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U U U U G U C U A U U U G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.9 3.7 3.1 5.5 3.2 3.0 2.4 4.8 3.2 3.0 2.4 4.8 3.9 3.7 3.1 5.5 3.9 3.7 3.1 5.5 3.9 3.7 3.1 5.5 3.8 3.7 5.5 3.7 3.1 3.0 4.8 3.0 3.1 3.0 4.8 3.0 3.8 3.7 5.5 3.7 3.8 3.7 5.5 3.7 3.8 3.7 5.5 3.7 3.2 5.5 2.3 5.5 2.5 4.8 1.6 4.8 2.5 4.8 1.6 4.8 3.2 5.5 2.3 5.5 3.2 5.5 2.3 5.5 3.2 5.5 2.3 5.5 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U U U U G U C U A U U U G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.6 3.2 3.1 5.5 2.9 2.5 2.4 4.8 2.9 2.5 2.4 4.8 3.6 3.2 3.1 5.5 3.6 3.2 3.1 5.5 3.6 3.2 3.1 5.5 3.7 4.0 5.5 3.7 3.0 3.3 4.8 3.0 3.0 3.3 4.8 3.0 3.7 4.0 5.5 3.7 3.7 4.0 5.5 3.7 3.7 4.0 5.5 3.7 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 3.7 5.5 2.8 4.8 3.0 4.8 2.1 4.8 3.0 4.8 2.1 5.5 3.7 5.5 2.8 5.5 3.7 5.5 2.8 5.5 3.7 5.5 2.8 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U U U U G U C U A U U U G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 2.5 2.1 1.9 5.5 1.8 1.4 1.2 4.8 1.8 1.4 1.2 4.8 2.5 2.1 1.9 5.5 2.5 2.1 1.9 5.5 2.5 2.1 1.9 5.5 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 2.3 5.5 3.7 5.5 1.6 4.8 3.0 4.8 1.6 4.8 3.0 4.8 2.3 5.5 3.7 5.5 2.3 5.5 3.7 5.5 2.3 5.5 3.7 5.5 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U U U U G U C U A U U U G YU YU YU YU YU YU 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 4.0 3.4 5.5 3.7 3.3 2.7 4.8 3.0 3.3 2.7 4.8 3.0 4.0 3.4 5.5 3.7 4.0 3.4 5.5 3.7 4.0 3.4 5.5 3.7 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 3.2 5.5 2.7 4.8 2.5 4.8 2.0 4.8 2.5 4.8 2.0 5.5 3.2 5.5 2.7 5.5 3.2 5.5 2.7 5.5 3.2 5.5 2.7 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U G U G G G C G A G U G G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.9 3.7 3.1 5.5 3.2 3.0 2.4 4.8 3.2 3.0 2.4 4.8 3.9 3.7 3.1 5.5 3.9 3.7 3.1 5.5 3.9 3.7 3.1 5.5 3.8 3.7 5.5 3.7 3.1 3.0 4.8 3.0 3.1 3.0 4.8 3.0 3.8 3.7 5.5 3.7 3.8 3.7 5.5 3.7 3.8 3.7 5.5 3.7 3.2 5.5 2.3 5.5 2.5 4.8 1.6 4.8 2.5 4.8 1.6 4.8 3.2 5.5 2.3 5.5 3.2 5.5 2.3 5.5 3.2 5.5 2.3 5.5 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U G U G G G C G A G U G G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.6 3.2 3.1 5.5 2.9 2.5 2.4 4.8 2.9 2.5 2.4 4.8 3.6 3.2 3.1 5.5 3.6 3.2 3.1 5.5 3.6 3.2 3.1 5.5 3.7 4.0 5.5 3.7 3.0 3.3 4.8 3.0 3.0 3.3 4.8 3.0 3.7 4.0 5.5 3.7 3.7 4.0 5.5 3.7 3.7 4.0 5.5 3.7 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 3.7 5.5 2.8 4.8 3.0 4.8 2.1 4.8 3.0 4.8 2.1 5.5 3.7 5.5 2.8 5.5 3.7 5.5 2.8 5.5 3.7 5.5 2.8 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U G U G G G C G A G U G G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 2.5 2.1 1.9 5.5 1.8 1.4 1.2 4.8 1.8 1.4 1.2 4.8 2.5 2.1 1.9 5.5 2.5 2.1 1.9 5.5 2.5 2.1 1.9 5.5 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 2.3 5.5 3.7 5.5 1.6 4.8 3.0 4.8 1.6 4.8 3.0 4.8 2.3 5.5 3.7 5.5 2.3 5.5 3.7 5.5 2.3 5.5 3.7 5.5 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U G U G G G C G A G U G G YU YU YU YU YU YU 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 4.0 3.4 5.5 3.7 3.3 2.7 4.8 3.0 3.3 2.7 4.8 3.0 4.0 3.4 5.5 3.7 4.0 3.4 5.5 3.7 4.0 3.4 5.5 3.7 5.5 5.5 5.5 5.5 4.8 4.8 4.8 4.8 4.8 4.8 4.8 4.8 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 3.2 5.5 2.7 4.8 2.5 4.8 2.0 4.8 2.5 4.8 2.0 5.5 3.2 5.5 2.7 5.5 3.2 5.5 2.7 5.5 3.2 5.5 2.7