Data tables for
asymmetric interior loops of size 3
Free energies at
37 degrees for RNA
Data arrangement:
Y
------------------
(X) A
C G U
------------------
5' --> 3'
X
A
A
U U
YA
3' <-- 5'
(A) .
. . .
(C) .
. . .
(G) .
. . .
(U) .
. . .
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
A
A A C A G A U A G A U
U
U U G U C
U A U U U G
YA YA YA YA YA YA
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
3.9
3.7 3.1 5.5
3.2 3.0 2.4
4.8 3.2 3.0
2.4 4.8 3.9
3.7 3.1 5.5
3.9 3.7 3.1
5.5 3.9 3.7
3.1 5.5
3.8
3.7 5.5 3.7
3.1 3.0 4.8
3.0 3.1 3.0
4.8 3.0 3.8
3.7 5.5 3.7
3.8 3.7 5.5
3.7 3.8 3.7
5.5 3.7
3.2
5.5 2.3 5.5
2.5 4.8 1.6
4.8 2.5 4.8
1.6 4.8 3.2
5.5 2.3 5.5
3.2 5.5 2.3
5.5 3.2 5.5
2.3 5.5
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
A
A A C A G A
U A G A U
U
U U G U C U A U U U G
YC YC YC YC YC YC
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
3.6
3.2 3.1 5.5
2.9 2.5 2.4
4.8 2.9 2.5
2.4 4.8 3.6
3.2 3.1 5.5
3.6 3.2 3.1
5.5 3.6 3.2
3.1 5.5
3.7
4.0 5.5 3.7
3.0 3.3 4.8
3.0 3.0 3.3
4.8 3.0 3.7
4.0 5.5 3.7
3.7 4.0 5.5
3.7 3.7 4.0
5.5 3.7
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
5.5
3.7 5.5 2.8
4.8 3.0 4.8
2.1 4.8 3.0
4.8 2.1 5.5
3.7 5.5 2.8
5.5 3.7 5.5
2.8 5.5 3.7
5.5 2.8
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
A
A A C A G A U A G A U
U
U U G U C U A U
U U G
YG YG YG YG YG YG
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
2.5
2.1 1.9 5.5
1.8 1.4 1.2
4.8 1.8 1.4
1.2 4.8 2.5
2.1 1.9 5.5
2.5 2.1 1.9
5.5 2.5 2.1
1.9 5.5
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
2.3
5.5 3.7 5.5
1.6 4.8 3.0
4.8 1.6 4.8
3.0 4.8 2.3
5.5 3.7 5.5
2.3 5.5 3.7
5.5 2.3 5.5
3.7 5.5
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
A
A A C A G A U A G A U
U
U U G U C U A U U U G
YU YU YU YU YU YU
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
4.0
3.4 5.5 3.7
3.3 2.7 4.8
3.0 3.3 2.7
4.8 3.0 4.0
3.4 5.5 3.7
4.0 3.4 5.5
3.7 4.0 3.4
5.5 3.7
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
5.5
3.2 5.5 2.7
4.8 2.5 4.8
2.0 4.8 2.5
4.8 2.0 5.5
3.2 5.5 2.7
5.5 3.2 5.5
2.7 5.5 3.2
5.5 2.7
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
C
A C C C G C U C G C U
G
U G G G C G A G U G G
YA YA YA YA YA YA
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
3.2
3.0 2.4 4.8
2.3 2.2 1.1
4.0 2.4 2.2
1.6 4.0 3.2
3.0 2.4 4.8
3.2 3.0 2.4
4.8 3.2 3.0
2.4 4.8
3.1
3.0 4.8 3.0
2.3 2.2 4.0
2.2 2.3 2.2
4.0 2.2 3.1
3.0 4.8 3.0
3.1 3.0 4.8
3.0 3.1 3.0
4.8 3.0
2.5
4.8 1.6 4.8
1.7 4.0 0.8 4.0 1.7
4.0 0.8 4.0
2.5 4.8 1.6
4.8 2.5 4.8
1.6 4.8 2.5
4.8 1.6 4.8
4.8
4.8 4.8 4.8
4.0 4.0 4.0
4.0 4.0 4.0
4.0 4.0 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
C
A C C C G C U
C G C U
G
U G G G C G A G U G G
YC YC YC YC YC YC
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
2.9
2.5 2.4 4.8
2.1 1.7 1.6
4.0 2.1 1.7
1.6 4.0 2.9
2.5 2.4 4.8
2.9 2.5 2.4
4.8 2.9 2.5
2.4 4.8
3.0
3.3 4.8 3.0
2.2 2.5 4.0
2.2 2.2 2.5
4.0 2.2 3.0
3.3 4.8 3.0
3.0 3.3 4.8
3.0 3.0 3.3
4.8 3.0
4.8
4.8 4.8 4.8
4.0 4.0 4.0
4.0 4.0 4.0
4.0 4.0 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8
4.8
3.0 4.8 2.1 4.0 2.2
4.0 1.5 4.0
2.2 4.0 1.3
4.8 3.0 4.8
2.1 4.8 3.0
4.8 2.1 4.8
3.0 4.8 2.1
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
C
A C C C G C U C G C U
G
U G G G C G A G U
G G
YG YG YG YG YG YG
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
1.8
1.4 1.2 4.8
0.8 0.6 0.4
4.0 1.0 0.6
0.4 4.0 1.8
1.4 1.2 4.8
1.8 1.4 1.2
4.8 1.8 1.4
1.2 4.8
4.8
4.8 4.8 4.8
4.0 4.0 4.0
4.0 4.0 4.0
4.0 4.0 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8
1.6
4.8 3.0 4.8
0.8 4.0 2.2
4.0 0.8 4.0
2.2 4.0 1.6
4.8 3.0 4.8
1.6 4.8 3.0
4.8 1.6 4.8
3.0 4.8
4.8
4.8 4.8 4.8
4.0 4.0 4.0
4.0 4.0 4.0
4.0 4.0 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
C
A C C C G C U C G C
U
G
U G G G C G A G U G G
YU YU YU YU YU YU
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
4.8
4.8 4.8 4.8
4.0 4.0 4.0
4.0 4.0 4.0
4.0 4.0 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8
3.3
2.7 4.8 3.0
2.5 1.9 4.0
2.2 2.5 1.9 4.0 2.2
3.3 2.7 4.8
3.0 3.3 2.7
4.8 3.0 3.3
2.7 4.8 3.0
4.8
4.8 4.8 4.8
4.0 4.0 4.0
4.0 4.0 4.0
4.0 4.0 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8
4.8
2.5 4.8 2.0
4.0 1.7 4.0 1.2 4.0
1.7 4.0 1.2
4.8 2.5 4.8
2.0 4.8 2.5
4.8 2.0 4.8
2.5 4.8 2.0
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
G
A G C G G G U G G G U
C
U C G C C C A C U C G
YA YA YA YA YA YA
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
3.2
3.0 2.4 4.8
2.4 2.2 1.6
4.0 2.5 2.2
2.1 4.0 3.2
3.0 2.4 4.8
3.2 3.0 2.4
4.8 3.2 3.0
2.4 4.8
3.1
3.0 4.8 3.0
2.3 2.2 4.0
2.2 2.3 2.2
4.0 2.2 3.1
3.0 4.8 3.0
3.1 3.0 4.8
3.0 3.1 3.0
4.8 3.0
2.5
4.8 1.6 4.8
1.7 4.0 0.8 4.0 1.7
4.0 0.8 4.0
2.5 4.8 1.6
4.8 2.5 4.8
1.6 4.8 2.5
4.8 1.6 4.8
4.8
4.8 4.8 4.8
4.0 4.0 4.0
4.0 4.0 4.0
4.0 4.0 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G
U A C
G U A
C G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
G
A G C G G G U G G G U
C
U C G C C C A C U C G
YC YC YC YC YC YC
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
2.9
2.5 2.4 4.8
2.1 1.7 1.6
4.0 2.1 1.7
1.6 4.0 2.9
2.5 2.4 4.8
2.9 2.5 2.4
4.8 2.9 2.5
2.4 4.8
3.0
3.3 4.8 3.0
2.2 2.5 4.0
2.2 2.2 2.5
4.0 2.2 3.0
3.3 4.8 3.0
3.0 3.3 4.8
3.0 3.0 3.3
4.8 3.0
4.8
4.8 4.8 4.8
4.0 4.0 4.0
4.0 4.0 4.0
4.0 4.0 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8
4.8
3.0 4.8 2.1
4.0 2.2 4.0
1.3 4.0 2.2 4.0 1.2
4.8 3.0 4.8
2.1 4.8 3.0
4.8 2.1 4.8
3.0 4.8 2.1
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
G
A G C G G G U G G G U
C
U C G C C C A C U C G
YG
YG YG YG YG YG
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
1.8
1.4 1.2 4.8
1.0 0.6 0.4
4.0 1.2 0.6
0.4 4.0 1.8 1.4 1.2
4.8 1.8 1.4
1.2 4.8 1.8
1.4 1.2 4.8
4.8
4.8 4.8 4.8
4.0 4.0 4.0
4.0 4.0 4.0
4.0 4.0 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8
1.6
4.8 3.0 4.8
0.8 4.0 2.2
4.0 0.8 4.0 2.2 4.0
1.6 4.8 3.0
4.8 1.6 4.8
3.0 4.8 1.6
4.8 3.0 4.8
4.8
4.8 4.8 4.8
4.0 4.0 4.0
4.0 4.0 4.0
4.0 4.0 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C
G U A
C G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
G
A G C G G G U G G G U
C
U C G C C C A C U C G
YU YU YU YU YU YU
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
4.8
4.8 4.8 4.8
4.0 4.0 4.0
4.0 4.0 4.0
4.0 4.0 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8
3.3
2.7 4.8 3.0
2.5 1.9 4.0
2.2 2.5 1.9 4.0 2.2
3.3 2.7 4.8
3.0 3.3 2.7
4.8 3.0 3.3
2.7 4.8 3.0
4.8
4.8 4.8 4.8
4.0 4.0 4.0
4.0 4.0 4.0
4.0 4.0 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8
4.8
2.5 4.8 2.0
4.0 1.7 4.0
1.2 4.0 1.7
4.0 1.2 4.8 2.5
4.8 2.0 4.8
2.5 4.8 2.0
4.8 2.5 4.8
2.0
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
U
A U C U G U U U G U U
A
U A G A C A A A U A G
YA YA YA YA YA YA
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
3.9
3.7 3.1 5.5
3.2 3.0 2.4
4.8 3.2 3.0
2.4 4.8 3.9
3.7 3.1 5.5
3.9 3.7 3.1
5.5 3.9 3.7
3.1 5.5
3.8
3.7 5.5 3.7
3.1 3.0 4.8
3.0 3.1 3.0
4.8 3.0 3.8
3.7 5.5 3.7
3.8 3.7 5.5
3.7 3.8 3.7
5.5 3.7
3.2
5.5 2.3 5.5
2.5 4.8 1.6
4.8 2.5 4.8
1.6 4.8 3.2 5.5 2.3
5.5 3.2 5.5
2.3 5.5 3.2
5.5 2.3 5.5
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
U
A U C U G U U U G U U
A
U A
G A C A A A U A G
YC YC YC YC YC YC
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
3.6
3.2 3.1 5.5
2.9 2.5 2.4
4.8 2.9 2.5
2.4 4.8 3.6
3.2 3.1 5.5
3.6 3.2 3.1
5.5 3.6 3.2
3.1 5.5
3.7
4.0 5.5 3.7
3.0 3.3 4.8
3.0 3.0 3.3
4.8 3.0 3.7 4.0 5.5
3.7 3.7 4.0
5.5 3.7 3.7
4.0 5.5 3.7
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
5.5
3.7 5.5 2.8
4.8 3.0 4.8
2.1 4.8 3.0
4.8 2.1 5.5
3.7 5.5 2.8
5.5 3.7 5.5
2.8 5.5 3.7
5.5 2.8
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
U
A U
C U G U U U G U U
A
U A G A C A A A U A G
YG YG YG YG YG YG
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
2.5
2.1 1.9 5.5
1.8 1.4 1.2
4.8 1.8 1.4
1.2 4.8 2.5 2.1 1.9
5.5 2.5 2.1
1.9 5.5 2.5
2.1 1.9 5.5
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
2.3
5.5 3.7 5.5
1.6 4.8 3.0
4.8 1.6 4.8
3.0 4.8 2.3
5.5 3.7 5.5 2.3
5.5 3.7 5.5
2.3 5.5 3.7
5.5
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
U
A U C U G U U U G U U
A
U A G A
C A A A U A G
YU YU YU YU YU YU
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
4.0
3.4 5.5 3.7
3.3 2.7 4.8
3.0 3.3 2.7
4.8 3.0 4.0
3.4 5.5 3.7 4.0 3.4
5.5 3.7 4.0
3.4 5.5 3.7
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
5.5
3.2 5.5 2.7
4.8 2.5 4.8
2.0 4.8 2.5
4.8 2.0 5.5
3.2 5.5 2.7
5.5 3.2 5.5
2.7 5.5 3.2
5.5 2.7
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
G
A G C G G G U G G G U
U
U U G U C U A U U U G
YA YA YA YA YA YA
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
3.9
3.7 3.1 5.5
3.2 3.0 2.4
4.8 3.2 3.0
2.4 4.8 3.9
3.7 3.1 5.5 3.9 3.7
3.1 5.5 3.9
3.7 3.1 5.5
3.8
3.7 5.5 3.7
3.1 3.0 4.8
3.0 3.1 3.0
4.8 3.0 3.8
3.7 5.5 3.7
3.8 3.7 5.5
3.7 3.8 3.7
5.5 3.7
3.2
5.5 2.3 5.5
2.5 4.8 1.6
4.8 2.5 4.8
1.6 4.8 3.2
5.5 2.3 5.5
3.2 5.5 2.3
5.5 3.2 5.5
2.3 5.5
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
G
A G C G G G U G G G U
U
U U G U C U A U U U G
YC YC YC YC YC YC
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
3.6
3.2 3.1 5.5
2.9 2.5 2.4
4.8 2.9 2.5
2.4 4.8 3.6
3.2 3.1 5.5
3.6 3.2 3.1
5.5 3.6 3.2
3.1 5.5
3.7
4.0 5.5 3.7
3.0 3.3 4.8
3.0 3.0 3.3
4.8 3.0 3.7
4.0 5.5 3.7
3.7 4.0 5.5 3.7
3.7 4.0 5.5
3.7
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
5.5
3.7 5.5 2.8
4.8 3.0 4.8
2.1 4.8 3.0
4.8 2.1 5.5
3.7 5.5 2.8
5.5 3.7 5.5
2.8 5.5 3.7
5.5 2.8
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A C
G U A
C G U
A C G
U A C
G U A
C G U
A C G
U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
G
A G C
G G G U G G G U
U
U U G U C U A U U U G
YG YG YG YG YG YG
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
2.5
2.1 1.9 5.5
1.8 1.4 1.2
4.8 1.8 1.4
1.2 4.8 2.5
2.1 1.9 5.5
2.5 2.1 1.9 5.5 2.5
2.1 1.9 5.5
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
2.3
5.5 3.7 5.5
1.6 4.8 3.0
4.8 1.6 4.8
3.0 4.8 2.3
5.5 3.7 5.5
2.3 5.5 3.7
5.5 2.3 5.5
3.7 5.5
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
G
A G C G G G U G G G U
U
U U G U C
U A U U U G
YU YU YU YU YU YU
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
4.0
3.4 5.5 3.7
3.3 2.7 4.8
3.0 3.3 2.7
4.8 3.0 4.0
3.4 5.5 3.7
4.0 3.4 5.5
3.7 4.0 3.4
5.5 3.7
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
5.5
3.2 5.5 2.7
4.8 2.5 4.8
2.0 4.8 2.5
4.8 2.0 5.5
3.2 5.5 2.7
5.5 3.2 5.5
2.7 5.5 3.2
5.5 2.7
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U A
C G U
A C G
U A C
G U A
C G U
A C G
U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
U
A U C U G
U U U G U U
G
U G G G C G A G U G G
YA YA YA YA YA YA
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
3.9
3.7 3.1 5.5
3.2 3.0 2.4
4.8 3.2 3.0
2.4 4.8 3.9
3.7 3.1 5.5
3.9 3.7 3.1
5.5 3.9 3.7 3.1 5.5
3.8
3.7 5.5 3.7
3.1 3.0 4.8
3.0 3.1 3.0
4.8 3.0 3.8
3.7 5.5 3.7
3.8 3.7 5.5
3.7 3.8 3.7
5.5 3.7
3.2
5.5 2.3 5.5
2.5 4.8 1.6
4.8 2.5 4.8
1.6 4.8 3.2
5.5 2.3 5.5
3.2 5.5 2.3
5.5 3.2 5.5
2.3 5.5
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
U
A U C U G U U U G U U
G
U G G G C G A
G U G G
YC YC YC YC YC YC
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
3.6
3.2 3.1 5.5
2.9 2.5 2.4
4.8 2.9 2.5
2.4 4.8 3.6
3.2 3.1 5.5
3.6 3.2 3.1
5.5 3.6 3.2
3.1 5.5
3.7
4.0 5.5 3.7
3.0 3.3 4.8
3.0 3.0 3.3
4.8 3.0 3.7
4.0 5.5 3.7
3.7 4.0 5.5
3.7 3.7 4.0
5.5 3.7
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
5.5
3.7 5.5 2.8
4.8 3.0 4.8
2.1 4.8 3.0
4.8 2.1 5.5
3.7 5.5 2.8
5.5 3.7 5.5
2.8 5.5 3.7
5.5 2.8
Y
Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G U
A C G
U A C
G U A
C G U
A C G
U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
U
A U C U G U U U G U U
G
U G G G C G A G U G G
YG YG YG YG YG YG
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
2.5
2.1 1.9 5.5
1.8 1.4 1.2
4.8 1.8 1.4
1.2 4.8 2.5
2.1 1.9 5.5
2.5 2.1 1.9
5.5 2.5 2.1
1.9 5.5
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
2.3
5.5 3.7 5.5
1.6 4.8 3.0
4.8 1.6 4.8
3.0 4.8 2.3
5.5 3.7 5.5
2.3 5.5 3.7
5.5 2.3 5.5
3.7 5.5
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
Y Y Y Y Y Y
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
A
C G U
A C G
U A C
G U A
C G U
A C G
U A C
G U
------------------ ------------------ ------------------ ------------------ ------------------ ------------------
5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'
X X X X X X
U
A U C U G U U U G U U
G
U G G G C G A G U G G
YU YU YU YU YU YU
3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
4.0
3.4 5.5 3.7
3.3 2.7 4.8
3.0 3.3 2.7
4.8 3.0 4.0
3.4 5.5 3.7
4.0 3.4 5.5
3.7 4.0 3.4
5.5 3.7
5.5
5.5 5.5 5.5
4.8 4.8 4.8
4.8 4.8 4.8
4.8 4.8 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5 5.5
5.5 5.5
5.5
3.2 5.5 2.7
4.8 2.5 4.8
2.0 4.8 2.5
4.8 2.0 5.5
3.2 5.5 2.7
5.5 3.2 5.5
2.7 5.5 3.2
5.5 2.7