∆G°37 =
∆G°37(Watson-Crick Pairs) + ∆G°37(Hairpin Loop)
∆G°37 = ∆G°37(Watson-Crick Pairs) + ∆G°37(terminal mismatch) + ∆G°37 Hairpin initiation(6) + ΔG°37 penalty (all C loops)
∆G°37 = ∆G°37(CG followed by AU) + ∆G°37(AU followed by CG) + ∆G°37(CG followed by AU) + ∆G°37 AU end penalty+ ∆G°37(AU followed by CC) + ∆G°37 Hairpin initiation(6) + 6×A + B
∆G°37 =
–2.11 kcal/mol – 2.24 kcal/mol – 2.11 kcal/mol + 0.45 kcal/mol – 0.7 kcal/mol
+
5.4 kcal/mol + 6×0.3 kcal/mol + 1.6 kcal/mol
∆G°37 =
+2.1 kcal/mol