∆G°37 =
∆G°37(Watson-Crick Pairs) + ∆G°37(Hairpin Loop)
∆G°37 = ∆G°37(Watson-Crick Pairs) + ∆G°37(terminal mismatch) + ∆G°37(GA first mismatch) + ∆G°37 Hairpin initiation(4)
+ ∆G°37 Tetraloop Bonus(CgaaaG)
∆G°37 = ∆G°37(CG followed by AU) + ∆G°37(AU followed by CG) + ∆G°37(CG followed by CG) + ∆G°37(CG followed by GA) + ∆G°37(GA first mismatch) + ∆G°37 Hairpin initiation(4) + ∆G°37 Tetraloop Bonus(CgaaaG)
∆G°37 = –2.11 kcal/mol – 2.24 kcal/mol – 3.26 kcal/mol – 1.4 kcal/mol – 0.8 kcal/mol + 5.6 kcal/mol – 3.0 kcal/mol
∆G°37 =
–7.2 kcal/mol