∆G°37 =
∆G°37(Watson-Crick Pairs) + ∆G°37(Hairpin Loop)
∆G°37 = ∆G°37(Watson-Crick Pairs) + ∆G°37(terminal mismatch) + ∆G°37(GA first mismatch) + ∆G°37 Hairpin initiation(5)
∆G°37 = ∆G°37(CG followed by AU) + ∆G°37(AU followed by CG) + ∆G°37(CG followed by AU) + ∆G°37 AU end penalty+ ∆G°37(AU followed by GG) + ∆G°37(GA first mismatch) + ∆G°37 Hairpin initiation(5)
∆G°37 =
–2.11 kcal/mol – 2.24 kcal/mol – 2.11 kcal/mol + 0.45 kcal/mol – 0.8 kcal/mol
– 0.8 kcal/mol +
5.6 kcal/mol
∆G°37 =
–2.0 kcal/mol