∆G°37 = ∆G°37(Watson-Crick Pairs) + ∆G°37 intermolecular initiation + ∆G°37 AU end penalty + ∆G°37(Bulge Loop)
∆G°37 =
∆G°37(GC
followed by AU) +∆G°37 intermolecular initiation + ∆G°37 AU end penalty + ∆G°37 bulge initiation(3)
∆G°37 =
–2.35 kcal/mol + 4.09 kcal/mol + 0.45 kcal/mol
+
3.2 kcal/mol
∆G°37 =
+5.4 kcal/mol