∆G°37 = ∆G°37(Watson-Crick Pairs) + ∆G°37 intermolecular initiation + ∆G°37(Bulge Loop)
∆G°37 =
∆G°37(GC
followed by CG) + ∆G°37(CG
followed by CG) + ∆G°37 intermolecular initiation + ∆G°37 bulge initiation(1) + ∆G°37(CG followed by GC)
∆G°37 =
–3.42 kcal/mol – 3.26 kcal/mol + 4.09 kcal/mol + 3.8 kcal/mol
– 2.36 kcal/mol
∆G°37 =
–1.2 kcal/mol