Data Arangement: Y ------------------ (X) A C G U ------------------ 5' ==> 3' AX AY 3' <== 5' (A) . . . . (C) . . . . (G) . . . . (U) -0.7 -0.1 -0.7 -0.1 -------------------------------- STACKING ENTHALPIES : TERMINAL MISMATCHES AND BASE-PAIRS. Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' AX AX AX AX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . . -3.9 2.0 -3.5 2.0 . . . . . . . . . . . . -2.3 6.0 -2.3 -0.3 . . . . . . . . . . . . -3.1 2.0 -3.5 2.0 . . . . . . . . . . . . -2.3 4.6 -2.3 -1.7 Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' CX CX CX CX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . -9.1 -5.6 -5.6 -5.6 . . . . . . . . . . . . -5.7 -3.4 -5.7 -2.7 . . . . . . . . . . . . -8.2 -5.6 -9.2 -5.6 . . . . . . . . . . . . -5.7 -5.3 -5.7 -8.6 . . . . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' GX GX GX GX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . -5.2 -4.0 -5.6 -4.0 . . . . -3.4 2.0 -3.5 2.0 . . . . -7.2 0.5 -7.2 -4.2 . . . . -2.3 6.0 -2.3 -0.3 . . . . -7.1 -4.0 -6.2 -4.0 . . . . -0.6 2.0 -3.5 2.0 . . . . -7.2 -0.3 -7.2 -5.0 . . . . -2.3 4.6 -2.3 1.6 Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' UX UX UX UX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' -4.0 -6.3 -8.9 -6.3 . . . . -4.8 -6.3 -8.9 -6.3 . . . . -4.3 -5.1 -4.3 -1.8 . . . . -4.3 -5.1 -4.3 -1.8 . . . . -3.8 -6.3 -8.9 -6.3 . . . . 3.1 -6.3 -1.5 -6.3 . . . . -4.3 -1.4 -4.3 1.4 . . . . -4.3 -1.4 -4.3 1.4 . . . .