Parameter: |
ΔG°37 loop |
ΔH°loop |
|
| Initiation n = 4 | +1.1 ± 0.1 | –7.2 ± 1.1 | |
| Initiation n = 5 | +2.0 ± 0.1 | –6.8 ± 1.8 | |
| Initiation n = 6 | +2.0 ± 0.1 | –1.3 ± 1.2 | |
| Asymmetry | +0.6 ± 0.1 | +3.2 ± 0.7 | |
| AU/GU closure | +0.7 ± 0.1 | +5.0 ± 0.7 | |
| 1×(n–1) terminal mismatch | 0 | 0 | |
| 2×3 terminal mismatch: |
5'RA 3'YG |
0 | 0 |
| 5'YA 3'RG |
–0.5 ± 0.2 | –5.7 ± 3.8 | |
| 5'RG 3'YA |
–1.2 ± 0.1 | –10.9 ± 2.7 | |
| 5'YG 3'RA |
–1.1 ± 0.1 | –8.6 ± 1.9 | |
| G G |
–0.8 ± 0.2 | –9.0 ± 4.6 | |
| U U |
–0.4 ± 0.1 | –6.4 ± 2.5 | |
| other size terminal mismatch: | 5'A 3'G |
–0.8 ± 0.1 | –3.4 ± 1.3 |
| 5G 3'A |
–1.0 ± 0.1 | –7.6 ± 1.0 | |
| G G |
–1.2 ± 0.1 | +2.8 ± 2.4 | |
| U U |
–0.7 ± 0.1 | –5.8 ± 1.1 | |
Note: In the context of 2×3 internal loops, the orientation of the closing pair is considered, where R is a purine and Y is a pyrimidine.