Data tables for asymmetric interior loops of size 3

The Enthalpy at 37 degrees for RNA

Data arrangement:

Y

------------------

(X) A C G U

------------------

5' --> 3'

X

A A

U U

YA

3' <-- 5'

(A) . . . .

(C) . . . .

(G) . . . .

(U) . . . .

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

A A A C A G A U A G A U

U U U G U C U A U U U G

YA YA YA YA YA YA

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 7.8 7.8 7.8 2.8 8.5 2.8 8.5 2.8 2.8 2.8 2.8 7.8 13.5 7.8 13.5 7.8 7.8 7.8 7.8 7.8 13.5 7.8 13.5

13.5 13.5 13.5 3.4 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

A A A C A G A U A G A U

U U U G U C U A U U U G

YC YC YC YC YC YC

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 13.5 7.8 13.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5

13.5 13.5 13.5 3.4 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

A A A C A G A U A G A U

U U U G U C U A U U U G

YG YG YG YG YG YG

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

7.8 7.8 7.8 7.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 7.8 7.8 7.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8

13.5 13.5 13.5 3.4 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

A A A C A G A U A G A U

U U U G U C U A U U U G

YU YU YU YU YU YU

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 13.5 7.8 13.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5

3.4 3.4 3.4 3.4 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

C A C C C G C U C G C U

G U G G G C G A G U G G

YA YA YA YA YA YA

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

8.5 8.5 2.8 8.5 2.6 3.5 2.5 3.5 3.5 3.5 -2.3 3.5 8.5 8.5 2.8 8.5 8.5 8.5 2.8 8.5 8.5 8.5 2.8 8.5

8.5 8.5 8.5 8.5 1.6 3.5 3.5 3.5 3.5 3.5 3.5 3.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5

2.8 2.8 2.8 2.8 -1.6 3.5 -4.1 3.5 -2.3 -2.3 -2.3 -2.3 2.8 8.5 2.8 8.5 2.8 2.8 2.8 2.8 2.8 8.5 2.8 8.5

8.5 8.5 8.5 -1.6 3.5 3.5 3.5 -6.7 3.5 3.5 3.5 -6.7 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

C A C C C G C U C G C U

G U G G G C G A G U G G

YC YC YC YC YC YC

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

8.5 8.5 2.8 8.5 3.5 -2.5 -2.3 3.5 3.5 3.5 -2.3 3.5 8.5 8.5 2.8 8.5 8.5 8.5 2.8 8.5 8.5 8.5 2.8 8.5

8.5 8.5 8.5 8.5 3.5 7.8 3.5 3.5 3.5 3.5 3.5 3.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5

2.8 8.5 2.8 8.5 -2.3 3.5 -2.3 3.5 -2.3 3.5 -2.3 3.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5

8.5 8.5 8.5 -1.6 3.5 5.8 3.5 -5.8 3.5 3.5 3.5 -6.7 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

C A C C C G C U C G C U

G U G G G C G A G U G G

YG YG YG YG YG YG

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

2.8 2.8 2.8 2.8 -2.3 -2.3 -2.3 -2.3 -2.3 -2.3 -2.3 -2.3 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8

8.5 8.5 8.5 8.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5

2.8 2.8 2.8 2.8 -3.1 -2.3 -2.3 -2.3 -2.3 -2.3 -2.3 -2.3 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8

8.5 8.5 8.5 -1.6 3.5 3.5 3.5 -6.7 3.5 3.5 3.5 -6.7 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

C A C C C G C U C G C U

G U G G G C G A G U G G

YU YU YU YU YU YU

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

8.5 8.5 2.8 8.5 3.5 3.5 -2.3 3.5 3.5 3.5 -2.3 3.5 8.5 8.5 2.8 8.5 8.5 8.5 2.8 8.5 8.5 8.5 2.8 8.5

8.5 8.5 8.5 8.5 6.4 3.5 3.5 3.5 3.5 3.5 3.5 3.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5

2.8 8.5 2.8 8.5 -2.3 3.5 -2.3 3.5 -2.3 3.5 -2.3 3.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5

-1.6 -1.6 -1.6 -1.6 -6.7 -6.9 -6.7 -7.0 -6.7 -6.7 -6.7 -6.7 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

G A G C G G G U G G G U

C U C G C C C A C U C G

YA YA YA YA YA YA

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

8.5 8.5 8.5 8.5 3.5 3.5 3.5 3.5 6.9 3.5 2.4 3.5 8.5 8.5 8.5 8.5 6.9 8.5 2.4 8.5 8.5 8.5 8.5 8.5

8.5 8.5 8.5 8.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5

2.8 2.8 2.8 2.8 -2.3 3.5 -2.3 3.5 -2.3 -2.3 -2.3 -2.3 2.8 8.5 2.8 8.5 2.8 2.8 2.8 2.8 2.8 8.5 2.8 8.5

8.5 8.5 8.5 -1.6 3.5 3.5 3.5 -6.7 3.5 3.5 3.5 -6.7 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

G A G C G G G U G G G U

C U C G C C C A C U C G

YC YC YC YC YC YC

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

8.5 8.5 8.5 8.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5

8.5 8.5 8.5 8.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5

2.8 8.5 2.8 8.5 -2.3 3.5 -2.3 3.5 -2.3 3.5 -2.3 3.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5

8.5 8.5 8.5 -1.6 3.5 3.5 3.5 -6.7 3.5 3.5 3.5 -6.7 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

G A G C G G G U G G G U

C U C G C C C A C U C G

YG YG YG YG YG YG

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

2.8 2.8 2.8 2.8 -2.3 -2.3 -2.3 -2.3 -5.0 -2.3 -2.3 -2.3 2.8 2.8 2.8 2.8 -5.0 2.8 2.8 2.8 2.8 2.8 2.8 2.8

8.5 8.5 8.5 8.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5

2.8 2.8 2.8 2.8 -2.3 -2.3 -2.3 -2.3 -2.3 -2.3 -2.3 -2.3 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8

8.5 8.5 8.5 -1.6 3.5 3.5 3.5 -6.7 3.5 3.5 3.5 -6.7 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

G A G C G G G U G G G U

C U C G C C C A C U C G

YU YU YU YU YU YU

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

8.5 8.5 8.5 8.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5

8.5 8.5 8.5 8.5 3.5 3.5 3.5 3.5 3.5 1.3 3.5 3.5 8.5 8.5 8.5 8.5 8.5 1.3 8.5 8.5 8.5 8.5 8.5 8.5

2.8 8.5 2.8 8.5 -2.3 3.5 -2.3 3.5 -2.3 3.5 -2.3 3.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5

-1.6 -1.6 -1.6 -1.6 -6.7 -6.7 -6.7 -6.7 -6.7 -6.7 -6.7 -6.7 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

U A U C U G U U U G U U

A U A G A C A A A U A G

YA YA YA YA YA YA

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

13.5 13.5 7.8 13.5 8.5 8.5 2.8 8.5 8.5 8.5 2.8 8.5 13.5 13.5 7.8 13.5 13.5 13.5 7.8 13.5 13.5 13.5 7.8 13.5

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 7.8 7.8 7.8 2.8 8.5 2.8 8.5 2.8 2.8 2.8 2.8 7.8 13.5 7.8 13.5 7.8 7.8 7.8 7.8 7.8 13.5 7.8 13.5

13.5 13.5 13.5 3.4 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

U A U C U G U U U G U U

A U A G A C A A A U A G

YC YC YC YC YC YC

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

13.5 13.5 7.8 13.5 8.5 8.5 2.8 8.5 8.5 8.5 2.8 8.5 13.5 13.5 7.8 13.5 13.5 13.5 7.8 13.5 13.5 13.5 7.8 13.5

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 13.5 7.8 13.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5

13.5 13.5 13.5 3.4 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

U A U C U G U U U G U U

A U A G A C A A A U A G

YG YG YG YG YG YG

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

7.8 7.8 7.8 7.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 7.8 7.8 7.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8

13.5 13.5 13.5 3.4 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

U A U C U G U U U G U U

A U A G A C A A A U A G

YU YU YU YU YU YU

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

13.5 13.5 7.8 13.5 8.5 8.5 2.8 8.5 8.5 8.5 2.8 8.5 13.5 13.5 7.8 13.5 13.5 13.5 7.8 13.5 13.5 13.5 7.8 13.5

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 13.5 7.8 13.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5

3.4 3.4 3.4 3.4 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

G A G C G G G U G G G U

U U U G U C U A U U U G

YA YA YA YA YA YA

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 6.9 8.5 2.4 8.5 13.5 13.5 13.5 13.5 6.9 13.5 2.4 13.5 13.5 13.5 13.5 13.5

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 7.8 7.8 7.8 2.8 8.5 2.8 8.5 2.8 2.8 2.8 2.8 7.8 13.5 7.8 13.5 7.8 7.8 7.8 7.8 7.8 13.5 7.8 13.5

13.5 13.5 13.5 3.4 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

G A G C G G G U G G G U

U U U G U C U A U U U G

YC YC YC YC YC YC

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 13.5 7.8 13.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5

13.5 13.5 13.5 3.4 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

G A G C G G G U G G G U

U U U G U C U A U U U G

YG YG YG YG YG YG

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

7.8 7.8 7.8 7.8 2.8 2.8 2.8 2.8 -5.0 2.8 2.8 2.8 7.8 7.8 7.8 7.8 -5.0 7.8 7.8 7.8 7.8 7.8 7.8 7.8

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 7.8 7.8 7.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8

13.5 13.5 13.5 3.4 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

G A G C G G G U G G G U

U U U G U C U A U U U G

YU YU YU YU YU YU

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 1.3 8.5 8.5 13.5 13.5 13.5 13.5 13.5 1.3 13.5 13.5 13.5 13.5 13.5 13.5

7.8 13.5 7.8 13.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5

3.4 3.4 3.4 3.4 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

U A U C U G U U U G U U

G U G G G C G A G U G G

YA YA YA YA YA YA

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

13.5 13.5 7.8 13.5 8.5 8.5 2.8 8.5 8.5 8.5 2.8 8.5 13.5 13.5 7.8 13.5 13.5 13.5 7.8 13.5 13.5 13.5 7.8 13.5

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 7.8 7.8 7.8 2.8 8.5 2.8 8.5 2.8 2.8 2.8 2.8 7.8 13.5 7.8 13.5 7.8 7.8 7.8 7.8 7.8 13.5 7.8 13.5

13.5 13.5 13.5 3.4 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

U A U C U G U U U G U U

G U G G G C G A G U G G

YC YC YC YC YC YC

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

13.5 13.5 7.8 13.5 8.5 8.5 2.8 8.5 8.5 8.5 2.8 8.5 13.5 13.5 7.8 13.5 13.5 13.5 7.8 13.5 13.5 13.5 7.8 13.5

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 13.5 7.8 13.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5

13.5 13.5 13.5 3.4 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

U A U C U G U U U G U U

G U G G G C G A G U G G

YG YG YG YG YG YG

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

7.8 7.8 7.8 7.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 7.8 7.8 7.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 2.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8

13.5 13.5 13.5 3.4 8.5 8.5 8.5 -1.6 8.5 8.5 8.5 -1.6 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4 13.5 13.5 13.5 3.4

Y Y Y Y Y Y

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

A C G U A C G U A C G U A C G U A C G U A C G U

------------------ ------------------ ------------------ ------------------ ------------------ ------------------

5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3'

X X X X X X

U A U C U G U U U G U U

G U G G G C G A G U G G

YU YU YU YU YU YU

3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5'

13.5 13.5 7.8 13.5 8.5 8.5 2.8 8.5 8.5 8.5 2.8 8.5 13.5 13.5 7.8 13.5 13.5 13.5 7.8 13.5 13.5 13.5 7.8 13.5

13.5 13.5 13.5 13.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5 13.5

7.8 13.5 7.8 13.5 2.8 8.5 2.8 8.5 2.8 8.5 2.8 8.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5 7.8 13.5

3.4 3.4 3.4 3.4 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 -1.6 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4 3.4