Parameter

ΔG°37 (kcal/mol)

ΔH° (kcal/mol)

UU or GA first mismatch 0.9 ± 0.1 5.8 ± 1.27
GG first mismatch 0.8 ± 0.3 0.0
special GU closure 2.2 ± 0.2 14.8 ± 2.35
C3 loop +1.5 ± 0.5 +18.6 ± 5.66
All-C loop, A +0.3 ± 0.1 +3.4 ± 1.48
All-C loop, B +1.6 ± 0.9 +7.6 ± 9.57