X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' AX AX AX AX A C G U 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . . -4.9 -0.9 -5.5 -2.3 X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' CX CX CX CX A C G U 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . -9.0 -4.1 -8.6 -7.5 . . . . X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' GX GX GX GX A C G U 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . -7.4 -2.8 -6.4 -3.6 . . . . -4.9 -0.9 -5.5 -2.3 X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' UX UX UX UX A C G U 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' -5.7 -0.7 -5.8 -2.2 . . . . -5.7 -0.7 -5.8 -2.2 . . . . X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' A A A A AX CX GX UX 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . . -0.5 6.9 0.6 0.6 X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' C C C C AX CX GX UX 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . -1.6 0.7 -4.6 -0.4 . . . . X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' G G G G AX CX GX UX 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . -2.4 3.3 0.8 -1.4 . . . . -0.5 6.9 0.6 0.6 X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' U U U U AX CX GX UX 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 1.6 2.2 0.7 3.1 . . . . 1.6 2.2 0.7 3.1 . . . .