ΔG°37 (kcal/mol)

3' Dangling Ends (kcal/mol)

5'––>3'
AX
U

A C G U
–0.8 –0.5 –0.8 –0.6
5'––>3'
CX
G
A C G U
–1.7 –0.8 –1.7 –1.2
5'––>3'
GX
C
A C G U
–1.1 –0.4 –1.3 –0.6
5'––>3'
GX
U
A C G U
–0.8 –0.5 –0.8 –0.6
5'––>3'
UX
A
A C G U
–0.7 –0.1 –0.7 –0.1
5'––>3'
UX
G
A C G U
–0.7 –0.1 –0.7 –0.1

5' Dangling Ends (kcal/mol)

A
UX
3'<––5'

A C G U
–0.3 –0.1 –0.2 –0.2

C
GX
3'<––5'

A C G U
–0.2 –0.3 0.0 0.0
G
CX
3'<––5'
A C G U
–0.5 –0.3 –0.2 –0.1
G
UX
3'<––5'
A C G U
–0.3 –0.1 –0.2 –0.2
U
AX
3'<––5'
A C G U
–0.3 –0.3 –0.4 –0.2
U
GX
3'<––5'
A C G U
–0.3 –0.3 –0.4 –0.2

ΔH° (kcal/mol)

3' Dangling Ends (kcal/mol)

5'––>3'
AX
U

A C G U
–4.9 –0.9 –5.5 –2.3
5'––>3'
CX
G
A C G U
–9.0 –4.1 –8.6 –7.5
5'––>3'
GX
C
A C G U
–7.4 –2.8 –6.4 –3.6
5'––>3'
GX
U
A C G U
–4.9 –0.9 –5.5 –2.3
5'––>3'
UX
A
A C G U
–5.7 –0.7 –5.8 –2.2
5'––>3'
UX
G
A C G U
–5.7 –0.7 –5.8 –2.2

5' Dangling Ends (kcal/mol)

A
UX
3'<––5'

A C G U
–0.5 +6.9 +0.6 +0.6

C
GX
3'<––5'

A C G U
–1.6 +0.7 –4.6 –0.4
G
CX
3'<––5'
A C G U
–2.4 +3.3 +0.8 –1.4
G
UX
3'<––5'
A C G U
–0.5 +6.9 +0.6 +0.6
U
AX
3'<––5'
A C G U
+1.6 +2.2 +0.7 +3.1
U
GX
3'<––5'
A C G U
+1.6 +2.2 +0.7 +3.1