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Perkin Elmer Lambda25 UV/ VIS Spectrophotometer
Turning off UV/ VIS Spectrophotometer Lamps
E. coli Large Scale Induction of Protein Expression
Lysis of E. coli by Sonication
Purify a Protein with Acidic pI
Purify a Protein with Basic pI
Purify a Protein with Thrombin Cleavage Site
Selenomethionine Protein Purification
A280 Protein Concentration Determination
Laemmli Method for SDS-PAGE Gels and Buffers
Prepare Sigma glutathione agarose
Precision Protease Purification and Use
TEV Protease Purification and Use
SF1 Mystery Domain Purification
Full length histag-U2AF65 Purification
Full length GST-ATPase Purification
Electrophoretic Mobility Shift Assays
Adjusting PhosphoImager Image Quality
BIAcore T100 for kinetic or affinity measurements
Isothermal Titration Calorimetry (ITC)
Fluoromax-3 Polarizer Alignment
Fluorescence Anisotropy RNA Binding Assay
Equations for Fitting Fluorescence Titration Assay
Electrophoretic Mobility Shift Assay
Amino Acid Analysis to Confirm Concentration for ITC
Effect of Interdomain Linker on Kd
Dynamic Light Scattering (DLS)
Small Angle X-ray Data Collection at ALS (with Greg Hura's help)
Scaling Pr functions calculated from SAXS data
Remote Data Collection at SSRL
Making LB for Plates and Liquid Media
PCR Primer Design for Subcloning
Restriction Enzyme Digest of DNA Insert and Vector
Gel Purifying Your PCR Product
Overnight Cultures of successful transformants
Freezer stocks of transformed bacteria