Parameter:

ΔG°37 loop
(kcal/mol)
Initiation n = 4 +1.7 ± 0.51
Initiation n = 5 +1.8 ± 0.56
Initiation n = 6 +2.0 ± 0.23
Asymmetry +0.48 ± 0.04
AU/GU closure +0.65
1×(n-1) terminal mismatch 0
other size terminal mismatch: GA or AG –1.1
UU –0.7

Note: In the context of 1x(n-1) internal loops, i.e. those with a sungle unpaired nucleotide on one side, there are no first mismatch bonuses applied.