Parameter	ΔG°37 (kcal/mol)
UU or GA first mismatch	–0.8 ± 0.2
special GU closure	–2.2 ± 0.5
C3 loop	+1.4
All-C loop, A	+0.3 ± 0.1
All-C loop, B	+1.6 ± 0.3