Data Arangement: Y ------------------ (X) A C G U ------------------ 5' ==> 3' AX AY 3' <== 5' (A) . . . . (C) . . . . (G) . . . . (U) -0.7 -0.1 -0.7 -0.1 -------------------------------- STACKING ENTHALPIES : Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' AX AX AX AX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . . . . . -6.8 . . . . . . . . . . . . . . -11.4 . . . . . . . . . . . . . . -10.5 . -3.2 . . . . . . . . . . . . -9.4 . -8.8 . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' CX CX CX CX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . -10.4 . . . . . . . . . . . . . . -13.4 . . . . . . . . . . . . . . -10.6 . -5.6 . . . . . . . . . . . . -10.5 . -12.1 . . . . . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' GX GX GX GX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . -12.4 . . . . . . . -12.8 . . . . . . -14.9 . . . . . . . -12.6 . . . . . . -13.4 . -8.3 . . . . . -12.1 . -13.5 . . . . -11.4 . -12.6 . . . . . -8.8 . -14.6 . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' UX UX UX UX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . -7.7 . . . . . . . -7.0 . . . . . . -12.4 . . . . . . . -8.3 . . . . . . -10.4 . -7.0 . . . . . -5.6 . -9.3 . . . . -6.8 . -12.8 . . . . . -3.2 . -13.5 . . . . .