## ΔG° |
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## 3' Dangling Ends (kcal/mol) |
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5'––>3' |
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A | C | G | U |

–0.8 | –0.5 | –0.8 | –0.6 |

5'––>3' CX G |
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A | C | G | U |

–1.7 | –0.8 | –1.7 | –1.2 |

5'––>3' GX C |
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A | C | G | U |

–1.1 | –0.4 | –1.3 | –0.6 |

5'––>3' GX U |
|||

A | C | G | U |

–0.8 | –0.5 | –0.8 | –0.6 |

5'––>3' UX A |
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A | C | G | U |

–0.7 | –0.1 | –0.7 | –0.1 |

5'––>3' UX G |
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A | C | G | U |

–0.7 | –0.1 | –0.7 | –0.1 |

## 5' Dangling Ends (kcal/mol) |
|||

A |
|||

A | C | G | U |

–0.3 | –0.1 | –0.2 | –0.2 |

C |
|||

A | C | G | U |

–0.2 | –0.3 | 0.0 | 0.0 |

G CX 3'<––5' |
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A | C | G | U |

–0.5 | –0.3 | –0.2 | –0.1 |

G UX 3'<––5' |
|||

A | C | G | U |

–0.3 | –0.1 | –0.2 | –0.2 |

U AX 3'<––5' |
|||

A | C | G | U |

–0.3 | –0.3 | –0.4 | –0.2 |

U GX 3'<––5' |
|||

A | C | G | U |

–0.3 | –0.3 | –0.4 | –0.2 |

## ΔH° (kcal/mol) |
|||

## 3' Dangling Ends (kcal/mol) |
|||

5'––>3' |
|||

A | C | G | U |

–4.9 | –0.9 | –5.5 | –2.3 |

5'––>3' CX G |
|||

A | C | G | U |

–9.0 | –4.1 | –8.6 | –7.5 |

5'––>3' GX C |
|||

A | C | G | U |

–7.4 | –2.8 | –6.4 | –3.6 |

5'––>3' GX U |
|||

A | C | G | U |

–4.9 | –0.9 | –5.5 | –2.3 |

5'––>3' UX A |
|||

A | C | G | U |

–5.7 | –0.7 | –5.8 | –2.2 |

5'––>3' UX G |
|||

A | C | G | U |

–5.7 | –0.7 | –5.8 | –2.2 |

## 5' Dangling Ends (kcal/mol) |
|||

A |
|||

A | C | G | U |

–0.5 | +6.9 | +0.6 | +0.6 |

C |
|||

A | C | G | U |

–1.6 | +0.7 | –4.6 | –0.4 |

G CX 3'<––5' |
|||

A | C | G | U |

–2.4 | +3.3 | +0.8 | –1.4 |

G UX 3'<––5' |
|||

A | C | G | U |

–0.5 | +6.9 | +0.6 | +0.6 |

U AX 3'<––5' |
|||

A | C | G | U |

+1.6 | +2.2 | +0.7 | +3.1 |

U GX 3'<––5' |
|||

A | C | G | U |

+1.6 | +2.2 | +0.7 | +3.1 |